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  • Molecular interactions among gametes and environment - Prof. Enrico Dainese - a.a. 2016/2017

    Born on September 28th, 1967, in Padova (Italy). Married with two children. Master's degree in Biological Sciences (University of Padova), title of PhD in “Biochemistry and biophysics applied to evolutionary biology” (University of Padova). Research activity at the University Paris-Sud (France) and University of Mainz (Germany). From 2000 to 2004 he was Researcher in Biochemistry at the Faculty of Veterinary Medicine. Since 2005 to the present he is Associate Professor in Biochemistry, Faculty of Biosciences, University of Teramo. From 2013 to 2015 he was President of the degree course in Biotechnology. Since 2014 he is coordinator of the Biochemistry and Molecular Biology unit of Research. Member of the Academic Board of the PhD in "Molecular and Cellular Biotechnology". In April 2015 he obtained the National Scientific Qualification as Full Professor in General Biochemistry and Clinical Biochemistry. Current teaching activities: "Structural and functional biochemistry"; "Nutritional and applied biochemistry". "Molecular interactions among gametes and environment". Scientific interests: 1. Structure and function of metalloproteins and membrane proteins; 2. biochemistry of bioactive lipids and nutraceutics involved in inflammatory and neurodegenerative diseases, and cancer; 3. development of new methodologies for the analysis of genetically modified organisms in food. Member of the editorial board of “Frontiers in Membrane Physiology and Biophysics”, and Associate Editor of the the US journal “Biotechnology and Applied Biochemistry”. http://scholar.google.it/citations?user=SLHpKr4AAAAJ&hl=it&oi=ao

     

    INFO ABOUT THE COURSE

    OBJECTIVES OF THE COURSE

    • Knowledge and understanding: The course provides, through lectures in English, a complete and updated of the molecular interactions that gametes put in place with the natural and artificial environment (membranes, culture media, media for cell cryopreservation and tissues, storage system, bioactive lipids). In addition, the course will focus on to the protein/protein and protein/lipid interactions that can give a functional modulation. Information about the microenvironment of biological membranes and their cellular functional changes will also be transferred to the student. The course is also aimed at the study of the physical principles that are at the basis of the freezing phenomenon. Understanding the different biological responses to low temperatures by biomolecules, cells and tissues, with particular attention to reproductive system. In this context, the main bioinformatics tools useful in the evaluation of interactions and structure/function relationships in biomolecules will be presented. In this way the student will acquire the ability to operate in three different and complementary contexts: in vivo, in vitro and in silico.
    • Applying knowledge and understanding: At the end of the course it will be evaluated:
      - Theoretical knowledge of the subject, by means of an oral examination;
      - The ability to apply theoretical knowledge and the main laboratory operations and the use of the available software for molecular interactions analyses using real practical tests and / or simulated.
    • Making judgements: During the practical lessons the student's ability to develop experimental protocols, to apply them properly, to collect and analyze the data correctly will be evaluated. Particular attention will be paid to the student's ability of critically comparing results with the available literature.
    • Communication: The communication skills of the student is specifically evaluated as follows:
      - Ongoing, throughout the course. The large amount of guided practice activities and the favorable ratio teachers / students will allow a continuous dialogue between students and teacher.
      - At the final examination.
      In particular students will be evaluated for their ability to communicate with a scientific and rigorous language.
    • Learning skills: The organization of the course in the three-weeks modules allows students to check their knowledge before moving on to the next module. In this way teachers are able to modulate the deepening of concepts given to the needs of the individual and class.
      The tight integration between lectures and practical activity allows teachers of the degree program to individually follow students. Using this approach the teachers can reach the final target uniformly training the highest possible number of students.


    PREREQUISITE AND PREPARATORY
    • Prerequisite: There are no prerequisites
    • Preparatory: No

    DUBLIN DESCRIPTORS FOR UNIT


    UNIT 1: Physicochemical principles and thermodynamics of the freezing of water solutions.

    • Knowledge and understanding: Chemical and physical structure of membrane lipids, effects of low temperatures on biological membranes and their role in the biochemical function at the cellular level.
    • Applying knowledge and understanding: Role of the cellular microenvironment and ligand/protein, protein/protein and protein/lipid interactions in the modulation of trafficking, signaling and cellular metabolic function.
    • Making judgements: During the practical lessons the student's ability to develop experimental protocols, to apply them properly, to collect and analyze the data correctly will be evaluated. Particular attention will be paid to the student's ability of critically comparing results with the available literature.
    • Communication: The communication skills of the student is specifically evaluated as follows:
      - Ongoing, throughout the course. The large amount of guided practice activities and the favorable ratio teachers / students will allow a continuous dialogue between students and teacher.
      - At the final examination.
      In particular students will be evaluated for their ability to communicate with a scientific and rigorous language.
    • Learning skills: The organization of the course in the three-weeks modules allows students to check their knowledge before moving on to the next module. In this way teachers are able to modulate the deepening of concepts given to the needs of the individual and class.
      The tight integration between lectures and practical activity allows teachers of the degree program to individually follow students. Using this approach the teachers can reach the final target uniformly training the highest possible number of students.


    UNIT 2: Description of key biochemical mechanisms involved in molecular interactions
    • Knowledge and understanding: Description of key biochemical mechanisms involved in the physicochemical and oxidative stress at the cellular level.
    • Applying knowledge and understanding: Effects of low temperatures on structure and function of proteins, biological membranes and metabolic enzymes/pathways. Biochemical strategies and molecular mechanisms of cryopreservation of biological material.
    • Making judgements: Design and development of synthetic lipid membranes (reconstituted microvesicles also from lipid mixtures ex vivo) and theirr use in biotechnology.
      All the topics will be treated both in lectures and in laboratory activity.
    • Communication: The communication skills of the student is specifically evaluated as follows:
      - Ongoing, throughout the course. The large amount of guided practice activities and the favorable ratio teachers / students will allow a continuous dialogue between students and teacher.
      - At the final examination.
      In particular students will be evaluated for their ability to communicate with a scientific and rigorous language.
    • Learning skills: The organization of the course in the three-weeks modules allows students to check their knowledge before moving on to the next module. In this way teachers are able to modulate the deepening of concepts given to the needs of the individual and class.
      The tight integration between lectures and practical activity allows teachers of the degree program to individually follow students. Using this approach the teachers can reach the final target uniformly training the highest possible number of students.

    COURSE BOOKS

    Life in the Frozen State

    • Author: Edited by Barry J. Fuller, Nick Lane, and Erica E. Benson
    • Edition: CRC Press, 2004, US
    • Pages: Print ISBN: 978-0-415-24700-9
    • Link ebook: DOI: 10.1201/9780203647073.ch2

    RESEARCH MATERIAL

    Modulation of the Endocannabinoid System by Lipid Rafts

    • Author: Enrico Dainese, Sergio Oddi, Monica Bari, and Mauro Maccarrone
    • Review: Current Medicinal Chamistry, year 14, 2007
    • Pages: 2702-2715
    • Link ebook: available at the portal of E-learning

    Interaction of Endocannabinoid Receptors with Biological Membranes

    INTERMEDIATE TESTS
    10 June practical and oral

    EVALUATION
    Class and laboratory attendance is strongly recommended. Oral exam for evaluation of technical and scientific knowledge and skills as well as the improvement of a professional and technical language.
    Project and research activities for product development with oral presentation of the final product in a dedicated session to evaluate communication, problem solving and team working skills (Dublin descriptors 3,4, 5).

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