Indice degli argomenti

  • Embryology - Prof. Pasqualino Loi - a.a. 2015/2016

    Name: Pasqualino

    Surname: Loi

    Born: Sardina (Italy), 15.10.1958

    Status: separated, two children, Cristina e Pietro Loi.

    Professional Preparation

    1980-1986- DVM Veterinary Medicine, Sassari University (Italy)

    1987-1990, PhD Reproductive Physiology, Sassari University (Italy)

    1990-1991, Post Doc in experimental embryology, Institute for Zootechnics, Olmedo, Sassari, Italy

    1992-1994, Post Doctoral Fellow Roslin Institute, UK

    September 1995 – EMBO Fellow, University of Newcastle Upon-Tyne, UK,

    1995-October 2000, research scientist, Institute for Zootechnics, Olmedo, Sassari, Italy
    November 2000 – Present: Associate Professor in Animal Physiology, University of Teramo (Italy)
    My main Scientific interest has always been Developmental Biology and particularly all aspects of Biotechnologies applicable on embryos; in vitro maturation and fertilization, in vitro embryo culture, embryo freezing, embryo biopsy and sexing by PCR, embryonic manipulation with special regard to nuclear transfer (cloning). My current area of research is nuclear reprogramming and the epigenetic modification of DNA induced on early embryos by cloning and other in vitro manipulations.
    My publications are available at:

    http://scholar.google.it/citations?user=VYcw_fAAAAAJ  

    INFO ABOUT THE COURSE


    OBJECTIVES OF THE COURSE

    • Knowledge and understanding: Topics: developmental biology of the main model organisms and mammals
      The frontal and practical lessons have been calibrated to provide a robust understanding of the mechanisms regulating early development
    • Applying knowledge and understanding: The background acquired on developmental biology will be applied for:i) execute basic micromanipulation techniques in the practical lessons; ii) to help students to select the topics most closer to their personal capacity/ambitions
    • Making judgements: The course foresees knowledge acquisition of ethically relevant issue, like the generation of totipotent/multipotent cells (embryonic, or otherwise induced multipotency - nuclear transfer), topics that touch sensible ethic issues. The students will learn the main ethical frame ruling scientific and pre/clinical application of the techniques acquired in the course.
    • Communication: The students will be invited to interact actively with the professors/teachers during frontal and practical lessons to implement their communication skills. to this extent, group of students will study and present in class a research paper selected by the tutor.
    • Learning skills: Students will acquire the skills to purse independent search in the field on developmental biology, should they wish to further explore arguments of pertinence of developmental biology.


    PREREQUISITE AND PREPARATORY
    • Prerequisite: Excellent knowledge of molecular biology of the cells and the main experimental approached to study cell function.
    • Preparatory: Excellent knowledge of anatomy/physiology of the reproductive tract in male and female; excellent knowledge of gamete biology.

    DUBLIN DESCRIPTORS FOR UNIT


    UNIT 1: Topics: developmental biology of the main model organisms and mammals

    • Knowledge and understanding: The frontal and practical lessons have been calibrated to provide a robust understanding of the mechanisms regulating early development
    • Applying knowledge and understanding: The background acquired on developmental biology will be applied for:i) execute basic micromanipulation techniques in the practical lessons; ii) to help students to select the topics most closer to their personal capacity/ambitions
    • Making judgements: The course foresees knowledge acquisition of ethically relevant issue, like the generation of totipotent/multipotent cells (embryonic, or otherwise induced multipotency - nuclear transfer), topics that touch sensible ethic issues. The students will learn the main ethical frame ruling scientific and pre/clinical application of the techniques acquired in the course.
    • Communication: The students will be invited to interact actively with the professors/teachers during frontal and practical lessons to implement their communication skills. to this extent, group of students will study and present in class a research paper selected by the tutor.
    • Learning skills: Students will acquire the skills to purse independent search in the field on developmental biology, should they wish to further explore arguments of pertinence of developmental biology.


    UNIT 2: Topics: reproductive biotechnologies
    • Knowledge and understanding: To get acquainted with the main tools used for the most invasive embryonic manipulation (cloning, transgenesis), and the basic mechanisms regulating them. Understanding the potential, limits and potential improvements of the reproductive biotechnologies
    • Applying knowledge and understanding: Having clear indication of the fields where the above embryo manipulation can be safely applied
    • Making judgements: The course foresees knowledge acquisition of ethically relevant issue, like the generation of totipotent/multipotent cells (embryonic, or otherwise induced multipotency - nuclear transfer, transgenic animals, etc), topics that touch sensible ethic issues. The students will learn the main ethical frame ruling scientific and pre/clinical application of the techniques acquired in the course.
    • Communication: The students will be invited to interact actively with the professors/teachers during frontal and practical lessons to implement their communication skills. to this extent, group of students will study and present in class a research paper selected by the tutor.
    • Learning skills: Students will acquire the skills to purse independent search in the field on developmental biology, should they wish to further explore arguments of pertinence of developmental biology.


    WEEKLY LESSONS

    • Monday hour 09.00-12.00
    • Tuesday hour 09.00-12.00 
    • Wednesday hour 09.00-12.00 
    • Thursday hour 09.00-12.00
    • Friday hour 09.00-12.00 

    COURSE BOOKS


    Fondamenti di Biologia dello Sviluppo

    • Author: Jonathan Slack
    • Edition: Zanichelli, 2007

    Introduzione alla Embriologia
    • Author: Boris I. Balinsky
    • Edition: Zanichelli, 2004

    Principles of Development
    • Author: Lewis Wolpert
    • Edition: Oxford University Press, 2002

    The mammalian preimplantation embryo
    • Author: Barry D. Bavister
    • Edition: Plenum Press, 1987

    Biologia dello sviluppo
    • Author: Scott F. Gilbert
    • Edition: Zanichelli, 2006

    RESEARCH MATERIAL

    The concepts of experimental embryology, From Eggs to Embryo




    • Link ebook: 

    INTERMEDIATE TESTS

    INTERMEDIATE TEST 1

    • Reference unit: 1
    • Date: 16/05/2016 alle 09:35
    • Type of test: test a risposte multiple, si/no

    INTERMEDIATE TEST 2
    • Reference unit: 2
    • Date: 20/05/2016 alle 09:35
    • Type of test: test

    EVALUATION
    test in itinere (quiz), esame finale (quiz)

  • Unit 1

    The course of embryology teaches students the basic mechanisms regulating embryonic and early foetal development, along with the classical research approaches to understand developmental biology. 

    The first lessons provides the students a backgorund on the early pioneers in developmental biology, focusing on the early attempts to understand the mechanisms leading to cell differentiation.