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  • Biomarkers in human reproduction - Prof. Cinzia Rapino - a.a. 2016/2017

    Prof. RAPINO CinziaPOSITION TITLE
    Assistant Professor in Biochemistry at the University of Teramo, Italy (contacts: crapino@unite.it, tel +39 0861 266842; fax +39 0861 266843).
    Education: 2001 University of Bologna, Italy M.S. in Pharmaceutical Chemistry and Technology; 2001-2005 University of Chieti "G. D’Annunzio", Chieti-Pescara (I) Ph.D. in Biomedical Sciences;2008 University of Teramo, Italy; 2005-2008 PostDoc position at the Laboratory of biochemistry and molecular biology directed by prof. Mauro Maccarrone, department of Biomedical Sciences, University of Teramo, Italy; 2008-2009 Research fellow at University Queen’s Belfast, Northern Ireland;

    Scientific interests:
    Published 41 full papers in international refereed journals (citations 751, h-index 17), on the following main topics:
    - Endocannabinoids and their role in mammalian reproduction;
    - Analysis of natural compounds (eg. nutraceuticals, bioactive lipids, etc.) and their metabolic pathways in foods of animal and vegetable origin;
    -Biochemistry of lipids, metabolic enzymes and receptors involved in physiological processes and inflammatory diseases, neurodegenerative and cancer;

    INFO ABOUT THE COURSE


    OBJECTIVES OF THE COURSE

    • Knowledge and understanding: The course allows the student to achieve a core of skills on the molecular and cellular mechanisms of the reproductive processes. This implies the assimilation of knowledge concerning: the biochemical nature of the different classes of compounds that act as intra- and inter-cellular messengers; the inter-relationships that allow the integration of different signals along pathways that lead to biological response and, finally, the theoretical basis and practical techniques to identify and quantify the various biological macromolecules.
    • Applying knowledge and understanding: At the end of the course, the theoretical knowledge of the topics treated in the course will be evaluated by a final oral examination, while the understanding of the biochemical and molecular techniques of application to the field of reproduction will be examined by a simulated laboratory test.
    • Making judgements: Making judgements:The lectures are designed to provide students with the basic knowledge that will help them achieve their investigational organizational skills in the laboratory practice. With the support of technical and scientific staff laboratory activities will be carried out by groups of students where each student puts into practice their analytical skills and critical judgment of the results obtained in real-time.
    • Communication: The communication skills of the student will be evaluated specifically through:
      - Written tests in progress at the end of each teaching unit;
      - Oral presentation (ppt) on a specific scientific article ;
      - Final examination.
    • Learning skills: The course is divided into two modules delivered intensively in a restricted period of 4 weeks. They contain theoretical and practical aspects that stimulate students to develop an autonomous capacity to resolve practical problems in the laboratory and that are indispensable for their future work experience. Students must demonstrate a development of good learning skills and critical analysis, that will be evaluated throughout the intermediate tests and the final examination.


    PREREQUISITE AND PREPARATORY
    • Prerequisite: Basic knowledge of the main concepts of Biochemistry and Molecular Biology are required. A one-week remedial course on biochemistry and molecular biology notions  is expected before the beginning of the course, generally in November and December.
    • Preparatory: None.

    DUBLIN DESCRIPTORS FOR UNIT


    UNIT 1: Signaling pathway in reproduction 1

    • Knowledge and understanding: The course allows the student to achieve a core of skills on the molecular and cellular mechanisms of the reproductive processes. This implies the assimilation of knowledge concerning: the biochemical nature of the different classes of compounds that act as intra- and inter-cellular messengers; the inter-relationships that allow the integration of different signals along pathways that lead to biological response and, finally, the theoretical basis and practical techniques to identify and quantify the various biological macromolecules.
    • Applying knowledge and understanding: At the end of the course, the theoretical knowledge of the topics treated in the course will be evaluated by a final oral examination, while the understanding of the biochemical and molecular techniques of application to the field of reproduction will be examined by a simulated laboratory test.
    • Making judgements: Making judgements:The lectures are designed to provide students with the basic knowledge that will help them achieve their investigational organizational skills in the laboratory practice. With the support of technical and scientific staff laboratory activities will be carried out by groups of students where each student puts into practice their analytical skills and critical judgment of the results obtained in real-time.
    • Communication: The communication skills of the student will be evaluated specifically through:
      - Written tests in progress at the end of each teaching unit;
      - Oral presentation (ppt) on a specific scientific article;
      - Final examination.
    • Learning skills: The course is divided into two modules delivered intensively in a restricted period of 4 weeks. They contain theoretical and practical aspects that stimulate students to develop an autonomous capacity to resolve practical problems in the laboratory and that are indispensable for their future work experience. Students must demonstrate a development of good learning skills and critical analysis, that will be evaluated throughout the intermediate tests and the final examination.


    UNIT 2: Signaling pathway in reproduction 2
    • Knowledge and understanding: The course allows the student to achieve a core of skills on the molecular and cellular mechanisms of the reproductive processes. This implies the assimilation of knowledge concerning: the biochemical nature of the different classes of compounds that act as intra- and inter-cellular messengers; the inter-relationships that allow the integration of different signals along pathways that lead to biological response and, finally, the theoretical basis and practical techniques to identify and quantify the various biological macromolecules.
    • Applying knowledge and understanding: At the end of the course, the theoretical knowledge of the topics treated in the course will be evaluated by a final oral examination, while the understanding of the biochemical and molecular techniques of application to the field of reproduction will be examined by a simulated laboratory test.
    • Making judgements: Making judgements:The lectures are designed to provide students with the basic knowledge that will help them achieve their investigational organizational skills in the laboratory practice. With the support of technical and scientific staff laboratory activities will be carried out by groups of students where each student puts into practice their analytical skills and critical judgment of the results obtained in real-time.
    • Communication: The communication skills of the student will be evaluated specifically through:
      - Written tests in progress at the end of each teaching unit;
      - Oral presentation (ppt) on a specific scientific article;
      - Final examination.
    • Learning skills: The course is divided into two modules delivered intensively in a restricted period of 4 weeks. They contain theoretical and practical aspects that stimulate students to develop an autonomous capacity to resolve practical problems in the laboratory and that are indispensable for their future work experience. Students must demonstrate a development of good learning skills and critical analysis, that will be evaluated throughout the intermediate tests and the final examination.


    UNIT 3: Signal molecules in reproduction 1
    • Knowledge and understanding: The course allows the student to achieve a core of skills on the molecular and cellular mechanisms of the reproductive processes. This implies the assimilation of knowledge concerning: the biochemical nature of the different classes of compounds that act as intra- and inter-cellular messengers; the inter-relationships that allow the integration of different signals along pathways that lead to biological response and, finally, the theoretical basis and practical techniques to identify and quantify the various biological macromolecules.
    • Applying knowledge and understanding: At the end of the course, the theoretical knowledge of the topics treated in the course will be evaluated by a final oral examination, while the understanding of the biochemical and molecular techniques of application to the field of reproduction will be examined by a simulated laboratory test.
    • Making judgements: Making judgements:The lectures are designed to provide students with the basic knowledge that will help them achieve their investigational organizational skills in the laboratory practice. With the support of technical and scientific staff laboratory activities will be carried out by groups of students where each student puts into practice their analytical skills and critical judgment of the results obtained in real-time.
    • Communication: The communication skills of the student will be evaluated specifically through:
      - Written tests in progress at the end of each teaching unit;
      - Oral presentation (ppt) on a specific scientific article;
      - Final examination.
    • Learning skills: The course is divided into two modules delivered intensively in a restricted period of 4 weeks. They contain theoretical and practical aspects that stimulate students to develop an autonomous capacity to resolve practical problems in the laboratory and that are indispensable for their future work experience. Students must demonstrate a development of good learning skills and critical analysis, that will be evaluated throughout the intermediate tests and the final examination.


    UNIT 4: Signal molecules in reproduction 2
    • Knowledge and understanding: The course allows the student to achieve a core of skills on the molecular and cellular mechanisms of the reproductive processes. This implies the assimilation of knowledge concerning: the biochemical nature of the different classes of compounds that act as intra- and inter-cellular messengers; the inter-relationships that allow the integration of different signals along pathways that lead to biological response and, finally, the theoretical basis and practical techniques to identify and quantify the various biological macromolecules.
    • Applying knowledge and understanding: At the end of the course, the theoretical knowledge of the topics treated in the course will be evaluated by a final oral examination, while the understanding of the biochemical and molecular techniques of application to the field of reproduction will be examined by a simulated laboratory test.
    • Making judgements: Making judgements:The lectures are designed to provide students with the basic knowledge that will help them achieve their investigational organizational skills in the laboratory practice. With the support of technical and scientific staff laboratory activities will be carried out by groups of students where each student puts into practice their analytical skills and critical judgment of the results obtained in real-time.
    • Communication: The communication skills of the student will be evaluated specifically through:
      - Written tests in progress at the end of each teaching unit;
      - Oral presentation (ppt) on a specific scientific article;
      - Final examination.
    • Learning skills: The course is divided into two modules delivered intensively in a restricted period of 4 weeks. They contain theoretical and practical aspects that stimulate students to develop an autonomous capacity to resolve practical problems in the laboratory and that are indispensable for their future work experience. Students must demonstrate a development of good learning skills and critical analysis, that will be evaluated throughout the intermediate tests and the final examination.

    COURSE BOOKS

    Fondamenti di Biochimica

    • Author: D. Voet, J.G. Voet, C.W. Pratt
    • Edition: N. Zanichelli, Bologna

    Mediatori Lipidici di Rilevanza Biomedica
    • Author: Maccarrone Mauro; Gasperi Valeria; Bari Monica
    • Edition: Piccin, Padova

    RESEARCH MATERIAL

    Articles and lecture notes provided by the Teacher


    INTERMEDIATE TESTS

    INTERMEDIATE TEST 1

    • Reference unit: 1-2
    • Date: 
    • Type of test: written

    INTERMEDIATE TEST 2
    • Reference unit: 3
    • Date: 
    • Type of test: written

    INTERMEDIATE TEST 3
    • Reference unit: 4
    • Date: 
    • Type of test: written

    EVALUATION
    - Written tests in progress at the end of each teaching unit; 

    - Oral presentation (ppt) on a specific scientific article; 

    - Final examination. 

  • Questo argomento

    UNIT 1

    Signaling pathway in reproduction:

    Cell signaling: cell-cell communication and cell-environment communication: types of segnals

  • UNIT 2

    Signaling pathway in reproduction: 
    Cell surface and intracellular receptors
    Signal transduction and second messengers
    G-protein-coupled receptors. Receptor tyrosine kinases
    Role of protein kinases and phosphatases in the cellular response
  • UNIT 3

    Signal molecules in reproduction


    Molecular Marker: definition and evaluation criteria

    Clinical development of a marker

    Signal molecules involved in reproduction: hormones, neurotransmitters and   elements of the immune system

    Molecular markers of female and male reproductive function

  • UNIT 4

    Signal molecules in reproduction


    Biomedical bibliographic research (MEDLINE: pubmed) of potential molecular markers to be used in the field of animal and human reproduction