Indice degli argomenti

  • Cryoconservation techniques of gametes - Prof. Nicola Bernabò - a.a. 2016/2017

    Currently: Associate Professor (SSD VET / 02), Veterinary Physiology, Faculty of Veterinary Medicine, University of Teramo.

    2004: PhD in "Endocrinology of Domestic Animals", University of Bologna

    2002: First level University Master in "Reproductive Biotechnology", University of Teramo

    1999: Degree in Veterinary Medicine, 110 cum laude, University of Pisa

    The research of Dr. Bernabo is focused on Reproductive Biology of Mammals. In particular it id focused on:
    - The adoption of computational models based on network theory (biological networks) to study the complex biological events, in particular in the acquisition of the fertilizing ability and the sperm-oocyte interaction.
    - Study of the effect of exposure to electromagnetic fields on reproductive function;
    - Study of the molecular mechanisms that control mammalian sperm capacitation.

    Altogether were made more than 70 research products including articles in international scientific journals, participation in conferences and book chapters, of which 48 are indexed to Scopus (http://www.scopus.com/authid/detail.uri ? AuthorID = 55907939900), with an H index = 13.
     

    INFO ABOUT THE COURSE


    OBJECTIVES OF THE COURSE

    • Knowledge and understanding: The course provides comprehensive and updated information about the molecular interactions that gametes put in place with the natural (the features male and female genitals) and artificial (culture media, media for cryopreservation of cells and tissues, storage systems) environment, with particular regard to the influence on them of the temperature.
      This knowledge will enable the student to address critically the different methodologies applied to the cultivation, handling and cryopreservation of both human and animal gametes.
    • Applying knowledge and understanding: At the end of the course will be evaluated:
      - Theoretical knowledge, by an oral examination;
      - The ability to apply theoretical knowledge by practical and / or simulated tests.
    • Making judgements: During the practical lessons the student's ability to develop experimental protocols, to apply them properly, and to collect and analyze the data correctly will be assessed . In particular, great attention will be paid to methodological rigor and the student's ability to turn an abstract problem in a scientific question.
    • Communication: The communication skills of the student is evaluated:
      - Ongoing: during the guided practice activities
      - During the exams.
      In particular it will be evaluated for their ability to communicate with a scientific and rigorous.
      language.
    • Learning skills: Student learning is evaluated by means of tests in progress and through a final oral examination.


    PREREQUISITE AND PREPARATORY
    • Prerequisite: It is required a strong background in anatomy, biochemistry and physiology of male and female gametes
    • Preparatory: None

    DUBLIN DESCRIPTORS FOR UNIT


    UNIT 1: Physiology of gametes at low temperature

    • Knowledge and understanding: The course provides comprehensive and updated information about the molecular interactions that gametes put in place with the natural (the features male and female genitals) and artificial (culture media, media for cryopreservation of cells and tissues, storage systems) environment, with particular regard to the influence on them of the temperature.
      This knowledge will enable the student to address critically the different methodologies applied to the cultivation, handling and cryopreservation of both human and animal gametes.
    • Applying knowledge and understanding: At the end of the course will be evaluated:
      - Theoretical knowledge, by an oral examination;
      - The ability to apply theoretical knowledge by practical and / or simulated tests.
    • Making judgements: During the practical lessons the student's ability to develop experimental protocols, to apply them properly, and to collect and analyze the data correctly will be assessed . In particular, great attention will be paid to methodological rigor and the student's ability to turn an abstract problem in a scientific question.
    • Communication: The communication skills of the student is evaluated:
      - Ongoing: during the guided practice activities
      - During the exams.
      In particular it will be evaluated for their ability to communicate with a scientific and rigorous.
      language.
    • Learning skills: Student learning is evaluated by means of tests in progress and through a final oral examination.


    UNIT 2: Cryopreservation of gametes
    • Knowledge and understanding: The course provides comprehensive and updated information about the molecular interactions that gametes put in place with the natural (the features male and female genitals) and artificial (culture media, media for cryopreservation of cells and tissues, storage systems) environment, with particular regard to the influence on them of the temperature.
      This knowledge will enable the student to address critically the different methodologies applied to the cultivation, handling and cryopreservation of both human and animal gametes.
    • Applying knowledge and understanding: At the end of the course will be evaluated:
      - Theoretical knowledge, by an oral examination;
      - The ability to apply theoretical knowledge by practical and / or simulated tests.
    • Making judgements: During the practical lessons the student's ability to develop experimental protocols, to apply them properly, and to collect and analyze the data correctly will be assessed . In particular, great attention will be paid to methodological rigor and the student's ability to turn an abstract problem in a scientific question.
    • Communication: In particular it will be evaluated for their ability to communicate with a scientific and rigorous.
      language.
    • Learning skills: Student learning is evaluated by means of tests in progress and through a final oral examination.


    UNIT 3: Introduction to computational modeling in cryobiology
    • Knowledge and understanding: The course provides comprehensive and updated information about the molecular interactions that gametes put in place with the natural (the features male and female genitals) and artificial (culture media, media for cryopreservation of cells and tissues, storage systems) environment, with particular regard to the influence on them of the temperature.
    • Applying knowledge and understanding: At the end of the course will be evaluated:
      - Theoretical knowledge, by an oral examination;
      - The ability to apply theoretical knowledge by practical and / or simulated tests.
    • Making judgements: During the practical lessons the student's ability to develop experimental protocols, to apply them properly, and to collect and analyze the data correctly will be assessed . In particular, great attention will be paid to methodological rigor and the student's ability to turn an abstract problem in a scientific question.
    • Communication: In particular it will be evaluated for their ability to communicate with a scientific and rigorous.
      language.
    • Learning skills: Student learning is evaluated by means of tests in progress and through a final oral examination.

    COURSE BOOKS

    The lesson slides will be mailny used as bibliographic reference

    RESEARCH MATERIAL

    Cellular Thermodynamics

    • Author: Joe Wolfe
    • Review: ENCYCLOPEDIA OF LIFE SCIENCES, 2002

    Potential Importance of Vitrification in Reproductive Medicine
    • Author: Juergen Liebermann, Frank Nawroth, Vladimir Isachenko, Evgenia Isachenko, Gohar Rahimi, and Michael J. Tucker
    • Review: BIOLOGY OF REPRODUCTION 67, 2002
    • Pages: 1671–1680

    INTERMEDIATE TESTS

    INTERMEDIATE TEST 1

    • Reference unit: 1
    • Date: at the end of reference unit
    • Type of test:

    INTERMEDIATE TEST 2
    • Reference unit: 2
    • Date: at the end of reference unit
    • Type of test:

    EVALUATION
    Multiple choice tests and oral exam

  • Unit II - Cryopreservation of gametes

  • Unit III - Introduction to computational modeling in cryobiology

  • Video Freezing Dynamics of Water

    • Video Supercooled Water Ice Crystal Formation

      • tutorial Cytoscape 2.8.2 I

        • tutorial Cytoscape 2.8.2 II

          scelta del layout

          analisi della topologia della rete con Network Analyzer

          funzione Visualize Parameters 

          • tutorial Cytoscape 2.8.2 III

            VizMapper

            attributi 

            • tutorial BioLayout Express 3D

              BioLayout Express 3D

              • tutorial Pathway Commons

                accesso a banche dati on-line

                esempio su come scaricare ed analizzare i dati da Pathway Commons

                • rete random: The evolution of the G(n,p) random graph.