LAB COURSE
Biotechnology - 1st year
Section outline
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Introduction to Lab safety and biological risk
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Optical microscopy. Morphological observations. Gram staining
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Viable plate count. Preparation of decimal dilutions. Spread plating.
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Viable plate count results. Isolation streaking method for pure culture. Catalase assay
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Biochemical identification by API kit: application and interpretation
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Genetic identification by 16s rRNA gene sequencing: electropherogram, BLAST NCBI, sequence annealing, dendogram
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