TEACHING UNIT 2
Biotechnology - 1st year
Section outline
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Microbial Nutrition and culture media
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Microbial growth. CMD. Viable count. Embedding plate, spreader, smear. MPN.
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isolation, propagation and pure culture. Turbidometry and growth curve (batch culture). Mathematical data of microbial growth.
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Introduction to bioenergetics. Glycolysis. Fermentation and substrate-level phosphorylation. Krebs cycle. Generation of PMF. Oxidative phosphorylation. Energy balance.
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Factors that influence the growth of microorganisms. Effect of temperature. Effect of water availability, salt concentration and compatible solutes.
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Factors that influence the growth of microorganisms. Effects of pH and oxygen on microbial growth.
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