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Luisa GIOIA

Reproductive Biotechnologies - 1st year

Section outline

  • Unit 1: Oocyte maturation. _Laboratory of IVM and IVF: equipment and instrumentation. _Oocyte IVM in domestic animals

    UNIT1


    Introduction to the course and required prerequisites.


    Laboratories on In Vitro Maturation (IVM) and In Vitro Fertilization (IVF) techniques.


    Setting up a laboratory: basic equipment and facilities required for a laboratory of IVM and IVF.
 


    Oocyte IVM in domestic animals:


    - Tissue culture systems used for IVM.

- Tissue culture media used for IVM.

- Selection of ovaries retrieved from slaughterhouses.

- Morphological evaluation of ovarian follicles and selection of cumulus-oocyte complexes (COCs).

- Choosing and setting up of the best culture system for oocyte IVM in domestic animals.
- Assessment of IVM oocytes.