Unit 3: IVM and IVF procedures for in vitro embryo production in the ovine model. _Assisted Reproductive Techniques (ART): IVF in Humans.
Reproductive Biotechnologies - 1st year
Section outline
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UNIT 3
IVM and IVF procedures for in vitro embryo production in the ovine model
Principles and simulation of IVF techniques used in the laboratory for assisted reproduction
- Principles about human IVM and superovulation protocols.
- Culture media for oocyte retrieval (ovum pick-up), sperm capacitation and IVF.
- Morphological evaluation of COCs collected by an ovum pick-up.
- Preparation of microdroplets under mineral oil.
- Protocols for sperm capacitation and IVF in the human model.
- Morphological evaluation of zygotes obtained by IVF.
- Introduction to assessment of early in vitro embryo development