Fisiologia dei sistemi - Prof. Pasqualino Loi - a.a. 2022/2023
Biotecnologie - 3° anno
ATTENZIONE: i contenuti di questo corso sono relativi all'anno accademico 2022/2023
Indice degli argomenti
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Family name: Loi First name:Pasqualino; ORCID: 0000-0003-4631-7663 Date of birth: 15th October 1958
EDUCATION
1991 PhD in Physiology of Reproduction, University of Sassari, Italy
1986 Degree in Veterinary Medicine, University of Sassari, Italy
CURRENT POSITION(S)
2019 January-present: Full Professor, Faculty of Veterinary, University of Teramo, Italy
PREVIOUS POSITIONS
2000 - 2018: Associate Professor, Faculty of Veterinary, University of Teramo, Italy
1994 - 2000 October: Senior Scientist, Institute of Zootechnic, Sardinia, Italy
1992 - 1994 Post Doc; Roslin Institute, Edinburgh, Scotland, UK
FELLOWSHIPS AND AWARDS
1994 EMBO Fellow, University of Newcastle upon Tyne, UK
2017 Japanese Society for the Promotion of Science (JSPS) Fellow, Riken Institute, Tsukuba, Japan
SUPERVISION OF GRADUATE STUDENTS AND POSTDOCTORAL FELLOWS
Under graduate students of Veterinary Sciences and Biotechnology of Reproduction (n° 23); PhD students (n°15); Post Docs: (n° 9)
2005 External Evaluator of the PhD program in the Polish Academy of Science, Poland 2008
External Evaluator of the PhD program in Nottingham University, UK
2017 External Evaluator of the PhD program in Bologna University, Italy 2016 External Evaluator of the PhD program in Milan University, Italy;
ORGANISATION OF SCIENTIFIC MEETINGS (last 5 years)
Member of the Scientific and Organizing Committee: International Congress on Animal Reproduction (ICAR), Bologna, June 2022, Italy
Member of the Scientific and Organizing Committee; Workshop "Micromanipulation of oocyte/embryos”, 33rd Meeting of the Association of Embryo Technologies in Europe, Bath, 8-9 September 2017; UK
Member of the Scientific and Organizing Committee; International Conference “Epigenetic Penetrance of Reproductive Technologies”, University of Teramo, 9-10 June 2016, project EU Horizon2020 "EraofArt", Twinning Action.
INSTITUTIONAL RESPONSIBILITIES
2009 - 2011: Scientific Advisory Board, EU ANIMBIOGEN, VII FP EU, Polish Academy of Sciences, Warsaw, Poland.
2007 - present: Review Board member for the European Science Foundation 2008-2011: Deputy of the EU COST “Gemini” Managing Committee –EU VII FP.
2004 - Present: PhD Committee in Veterinary Sciences, University of Teramo
2012 - Present: Member of the Steering Committee for the European Association of European Veterinary Education (EAVE)
2018 - present: Responsible person for students with disabilities of the Faculty of Veterinary Medicine in Teramo
2019 - present: President of the Committee for the Research Assessment, Faculty of Veterinary, University of Teramo.
2020 – present: European Research Council, Executive Agency (ERCEA), Seconded National Experts, Department B, Life Sciences, reserve list.
Editorial Board Member
Animal Science Papers and Reports. ISSN: 0860 4037
Cellular Reprogramming. ISSN: 2152- 4971
Journal of Reproduction and Development”. ISSN: 1348-4400
Frontiers in Cell and Developmental Biology: Electronic ISSN: 2296-634X
Frontiers in Physiology: Electronic ISSN: 1664-042X
Review, ad hoc
Ad hoc reviewer: Nature, Biology of Reproduction, Theriogenology, Gene, Trends in Biotechnology, Cloning and Stem Cells, Reproduction, Fertility and Development, Genomics, Reproduction in Domestic Animals, PLOS ONE, Scientific Reports, etc.
Referee for funding agencies
2007 –Referee for the European Research Council –remote evaluation - (ERC), VII FP EU
2010-2014 Referee for the Marie Curie Fellowship Program, VII FP EU
2011-2019 Referee for the Polish Academy of Sciences
2013 Referee for the Royal Society, UK
2013 Referee for the USA Israel Bi-National Scientific Program
2015-2019 Referee for the Cyprus Academy of Sciences
2017-to present Referee for the European Science Foundation
2018 Referee for the Lithuanian National Science Foundation
2018 Referee for the Medical Research Council, UK
2019 Referee for the Fonds Wetenschappelijk Onderzoek, Belgium
2018-to present Referee for the Dutch Science Foundation
2018-to present Referee for the Czech Academy of Sciences
2010-to present Referee for the Italian Minister of Research and University
2020-to present Referee for the Natural Sciences and Engineering Research Council of Canada.
MEMBERSHIPS OF SCIENTIFIC SOCIETIES
Italian Society of Veterinary Sciences (SISVET); Italian Association of Veterinary Physiologists, Steering Committee (SOFIVET; International Embryo Technology Association (IETS)
Granted Patents
Pasqualino Loi et al., Improved method for reconstructing a non-human animal embryo EP 3134532 A1 (WO2015162170A1)
Pasqualino Loi & Pietro Cappai. Process for reconstructing a non-human mammalian embryo by nuclear transfer using a heat-treated donor nucleus and preparing a non-human mammal US 7064245 B1; PCT/IT1999/00016
Invited presentations to internationally established conferences (last 5 years):
2021 International Embryo Technology Society (IETS) 47th Annual Conference, Lima, Peru.
2020 Ovarian Club, XIV Symposium, Paris, France.
2019 Ovarian Club, XIV Symposium, Paris, France.
2018 Ovarian Club, XIII Symposium, Paris, France.
2017 World Congress of Reproduction Biology (WCRB2017), Okinawa, Japan.
2017 Laudazio Prof. Galli: recipient of the AETE pioneer award 2017", 33rd Meeting of the Association of Embryo Technology in Europe (AETE). Bath, UK.
2016 VI International Symposium of Animal Biology of Reproduction. Campos do Jordao, SP, Brazil.
2015 International Northern White Rhino Working Group: Conservation by Cellular Technologies, Vienna,Austria.
2015 Cost Action Meeting Epigenetics and Peri-conception Environment, Hersonissos, Crete, Greece.
2015 International Symposium of Functional Genomics Piacenza, Piacenza, Italy.
2015 International Embryo Technology Society (IETS) 41st Annual Conference, Versailles, France.
Short Biographical Sketch
I started to work on embryo micromanipulation, 30 years ago on embryo multiplication by splitting and embryonic cells nuclear transfer during my PhD. Next I joined Keith Campbell and Ian Wilmut at the Roslin Institute, UK, as a Post Doc. The publications jointly delivered (Campbell, KHS Loi et al., Reviews of Reproduction, 1996; Campbell KHS, Loi P., Biol Reprod. 1994) prepared the ground for the production of the first sheep cloned from embryonic (Campbell et al., Nature 1996;380: 64) and adult cells (Wilmut et al., Nature 1997, 385: 810). After my work at Roslin, I continued to work on Somatic Cell Nuclear Transfer (SCNT), producing the first wild animal by cloning (Loi et al. Nature Biotechnology, 2001), even using dead cells (Loi et al., Biol Reprod. 2002), a breakthrough that has prepared the ground for cloning using lyophilized cells (Loi et al., PLOS ONE 2008), and paved the way to the most recent attempt to clone the mammoth (Yamagata et al., Sci Rep. 2019; 9:4050). The latest breakthrough of my research group is to improve nuclear reprogramming by conferring somatic cells the nuclear structure of a spermatid through the heterologous expression of protamine1 (Iuso et al., Cell Reports 2015; Czernik et al., Nature Protocols 2016; Palazzese et al., PLOS ONE, 2018).
Metrics - My H Index is 35 (Google Scholar), with 4250 citations. My H- index and number of citations may appear low; however, I was amongst the first researchers to work on nuclear transfer. For many years the number of groups worldwide on the topics was below 10 so it is not possible to have a high H-index in such circumstances, considering also that I publish on animal science, notoriously with low IF.
Latest Dissemination as influential Scientist in my field https://www.nature.com/news/endangered-species-sex-and-the-single-rhinoceros-1.10731 https://www.scientificamerican.com/article/scientists-successfully-c/ https://www.scientificamerican.com/article/spry-dolly-siblings-could-make-clone-skeptics-sheepishhttps://www.scientificamerican.com/article/cloning-endangered-animals/https://www.scientificamerican.com/article/rhinoceros-assisted-reproduction-rescue-sepcies http://www.nytimes.com/2016/07/27/science/dolly-the-sheep-clones.html?_r=0; https://doi.org/10.1038/nature.2012.10228
INFO SUL CORSO
OBIETTIVI GENERALI DEL CORSO
Conoscenza e capacità di comprensione: Il corso ha sempre avuto un ottimo livello di gradimento tra gli studenti. Le lezioni frontali sono organizzate in modo informale, finalizzate a suscitare interesse nello studente e a renderlo partecipe dell’affascinante disciplina delle tecniche di coltura cellulare e delle cellule staminali, anche in considerazione del grande interesse scientifico e applicativo soprattutto a carico di queste ultime. Le lezioni sono integrate da filmati che facilitano la memorizzazione di eventi biologici complessi. Un’attenzione particolare è rivolta a informare gli studenti sulle ricerche più recenti e sugli aspetti etici dell’impiego delle cellule staminali nella pratica clinica.
Capacità di applicare conoscenza e comprensione: La piena comprensione del funzionamento delle cellule e della biologia delle cellule staminali è possibile solo se si possiede una giusta conoscenza della fisiologia cellulare sostenuta da adeguate conoscenze di chimica, biochimica e citologia. Gli studenti sono quindi chiamati ad applicare allo studio delle cellule somatiche e staminali le conoscenze acquisite nei corsi precedenti.
Autonomia di giudizio: Applicherei questo parametro agli aspetti etici di alcune tecniche d’impiego delle cellule staminali, iniziando dal materiale di partenza (embrione verso cellule staminali somatiche –mesenchimali di diversa origine) per il loro isolamento. Gli studenti ricevono tutte le informazioni scientifiche e lo stato dell’arte più avanzato della materia per metterli in condizioni di giudicare vantaggi, rischi, e problemi derivanti dalle tecnologie cellulari affrontate nel corso.
Abilità comunicative: Il miglioramento delle capacità comunicative degli studenti è una priorità di questo corso. Per tale motivo gli studenti sono continuamente invitati a fornire un loro parere sugli argomenti trattati, incoraggiandoli a dare le loro interpretazioni di un dato meccanismo biologico.
Capacità di apprendimento: Le tematiche affrontate nel corso sono fortemente coinvolgenti e di grande attualità, come il caso celle cellule staminali. Le lezioni sono strutturate in modo tale che lo studente partecipa attivamente alla discussione, e questo va a vantaggio della facilità di apprendimento dei contenuti.
PREREQUISITI E PROPEDEUTICITA'
Prerequisiti: Essere iscritti al terzo anno di corso di Biotecnologie
Propedeuticità: Nessuna
INDICATORI DI DUBLINO
UNITA' DIDATTICA 1:
UNITA' DIDATTICA 2:
LIBRI DI TESTO
Culture of Animal Cells. A manual of basic technique
Autore: R. Ian Freshney
Edizione: Wiley-Blackwell, 2010
Lezione 1: chapter 1, chapter 4, 5 and 6
Lezione 2: chapter 9 and 10
Cryopreservazione: chapter 20
MATERIALE DI APPROFONDIMENTO
PROVE INTERMEDIE
PROVA INTERMEDIA 1
Unità didattica di riferimento: 1
Data:
Tipologia di prova: test a risposta multipla
ARGOMENTI PER IL PARZIALE I:
LEZIONI FRONTALI
Cell Culture
Atrezzatura di laboratorio
Medium composition
Cytotoxicity
Caratterrizzazione cellulare
Cryopreservazione
LABORATORIO
Coltura primaria
Caratterrizzazione cellulare
PROVA INTERMEDIA 2
Unità didattica di riferimento: 2
Data:
Tipologia di prova: test a risposta multipla
ARGOMENTI PER IL PARZIALE II:
LEZIONI FRONTALI:
Riprogramming, SCNT, stem cells (Luca)
LABORATORO
Cariotipo
Cells separation, Freezing, Counting
MODALITA' DI VALUTAZIONE
Test a risposta multipla
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L’esercitazione ha lo scopo di fornire allo studente le conoscenze di base per l’isolamento di cellule da tessuti al fine di allestire una coltura primaria.
L’allestimento di una coltura cellulare può avere innumerevoli applicazioni come la produzione di vaccini, l’isolamento di specifiche tipologie cellulari che si desidera studiare, analisi citogenetiche, sviluppo di terreni di coltura selettivi, ingegneria tissutale, test di citossicità.
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