Techniques for biomarkers identification in reproduction - Prof. Mariangela Pucci - a.y. 2021/2022
ATTENZIONE: i contenuti di questo corso sono relativi all'anno accademico 2021/2022
Indice degli argomenti
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Actual Position: Assistant Professor of Molecular Biology (BIO/11). MS in Biological Science at the University of L'Aquila, Italy; Ph.D. degree in Reproductive Biotechnology, at the University of Teramo, Italy; Post-doctoral Fellow at the Department of Biosciences and Nutrition, Karolinska Institute, Stockholm (SE).
Scientific interests:
- neurobiology and molecular mechanisms underlying human eating disorders and obesity;
- development of new model for human binge eating behaviors in the worm C. elegans;
- bioactive lipids and the molecular mechanisms of G protein-coupled receptor signalling.
Info about the course
Objectives of the course
• Knowledge and understanding: The course allows the student to achieve a core of skills on the molecular and cellular mechanisms of the reproductive processes. This implies the assimilation of knowledge concerning: the biochemical nature of the different classes of compounds that act as intra- and inter-cellular messengers; the inter-relationships that allow the integration of different signals along pathways that lead to biological response and, finally, the theoretical basis and practical techniques to identify and quantify the various biological macromolecules.
• Applying knowledge and understanding: At the end of the course, the theoretical knowledge of the topics treated in the course will be evaluated by a final oral examination, while the understanding of the biochemical and molecular techniques of application to the field of reproduction will be examined by a simulated laboratory test.
• Making judgements: The lectures are designed to provide students with the basic knowledge that will help them achieve their investigational organizational skills in the laboratory practice. With the support of technical and scientific staff laboratory activities will be carried out by groups of students where each student puts into practice their analytical skills and critical judgment of the results obtained in real-time.
• Communication: The communication skills of the student will be evaluated specifically through:- Written tests in progress at the end of each teaching unit;
- Oral presentation (ppt) on a specific scientific article ;
- Final examination.
• Learning skills: The course is divided into two modules delivered intensively in a restricted period of 4 weeks. They contain theoretical and practical aspects that stimulate students to develop an autonomous capacity to resolve practical problems in the laboratory and that are indispensable for their future work experience. Students must demonstrate a development of good learning skills and critical analysis, that will be evaluated throughout the intermediate tests and the final examination.
Prerequisite and Preparatory
• Prerequisite: General concepts of biochemistry and molecular biology.
• Preparatory: None.Dublin Descriptord For Unit
Unit 1:
Unit 2
Course Books
Endocannabinoid Signaling: Methods and Protocols
- Author: Mauro Maccarrone (ed.)
- Edition: Methods in Molecular Biology, vol. 1412, DOI 10.1007/978-1-4939-3539-0_24
Epigenetics
- Author: C. David Allis, Marie-Laure Caparros, Thomas Jenuwein, Danny Reinberg, editors.
- Edition: Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY.
Research articles and reviews provided by the Teacher.
Intermedie Test 1
• Reference unit: 1
• Date:
• Type of test: Written testIntermedie Test 2
• Reference unit: 2
• Date:
• Type of test: Written testEvaluation
- Written tests in progress at the end of each teaching unit;
- Oral presentation (ppt) on a specific scientific article;
- Final examination. -
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